More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| BCL2 binding component 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human PUMA(145-159aa ADDLNAQYERRRQEE), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BBC3 |
Protein | Bcl-2-binding component 3 |
Uniprot ID | Q9BXH1 |
Function | Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis. |
Tissue Specificity | Ubiquitously expressed. |
Sub-cellular localization | Mitochondrion. |
Sequence Similarities | Belongs to the Bcl-2 family. |
Aliases | BBC 3 antibody|BBC3 antibody|BBC3_HUMAN antibody|BCL 2 binding component 3 antibody|Bcl-2-binding component 3 antibody|BCL2 binding component 3 antibody|JFY 1 antibody|JFY-1 antibody|JFY1 antibody|p53 up regulated modulator of apoptosis antibody|p53 up-regulated modulator of apoptosis antibody|p53 Upregulated Modulator of Apoptosis antibody|PUMA alpha antibody|PUMA/JFY1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PUMA antibody, ASA-B1596, Western blotting
All lanes: Anti PUMA(ASA-B1596) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Predicted bind size: 21KD
Observed bind size: 21KD
All lanes: Anti PUMA(ASA-B1596) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Predicted bind size: 21KD
Observed bind size: 21KD
