More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| prostate tumor overexpressed 1 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human PTOV1 (385-414aa NFVNGIRRVIANQQQVLQRNLEQEQQQRGM), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PTOV1 |
Protein | Prostate tumor-overexpressed gene 1 protein |
Uniprot ID | Q86YD1 |
Function | May activate transcription. Required for nuclear translocation of FLOT1. Promotes cell proliferation. |
Tissue Specificity | Expressed in brain, heart, kidney, liver, placenta, skeletal muscle and small intestine. |
Sub-cellular localization | Cell membrane. Cytoplasm, perinuclear region. Nucleus. Note: Translocates from the cytoplasm to the nucleus at the onset of S-phase. Also localizes to lipid rafts. |
Sequence Similarities | Belongs to the Mediator complex subunit 25 family. PTOV1 subfamily. |
Aliases | ACID2 antibody|Activator interaction domain containing protein 2 antibody|Gcap3 antibody|prostate tumor overexpressed 1 antibody|Prostate tumor overexpressed gene 1 antibody|PTOV 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- PTOV1 antibody, ASA-B1595, Western blotting
All lanes: Anti PTOV1 (ASA-B1595) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Predicted bind size: 47KD
Observed bind size: 47KD
All lanes: Anti PTOV1 (ASA-B1595) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Predicted bind size: 47KD
Observed bind size: 47KD
