More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| prostaglandin E receptor 2 (subtype EP2), 53kDa | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human PTGER2 (281-308aa TIFAYMNETSSRKEKWDLQALRFLSINS), different from the related mouse sequence by four amino acids, and from the related rat sequence by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PTGER2 |
Protein | Prostaglandin E2 receptor EP2 subtype |
Uniprot ID | P43116 |
Function | Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle. |
Tissue Specificity | Placenta and lung. |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the G-protein coupled receptor 1 family. |
Aliases | EP 2 antibody|EP2 antibody|Human prostaglandin E2 receptor antibody|PE2R2 _HUMAN antibody| PGE receptor EP2 subtype antibody|PGE2 receptor EP2 subtype antibody|Prostaglandin E receptor 2 EP2 subtype antibody|Prostaglandin E receptor 2 subtype EP2 53kDa antibody| Prostaglandin E receptor 2 subtype EP2 antibody|Prostaglandin E2 receptor antibody| Prostaglandin E2 receptor EP2 subtype antibody|Prostanoid EP2 receptor antibody|PTGER 2 antibody|PTGER2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- PTGER2 antibody, ASA-B1594, Western blotting
All lanes: Anti PTGER2 (ASA-B1594) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti PTGER2 (ASA-B1594) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 40KD
Observed bind size: 40KD
