More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| prostate stem cell antigen | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human PSCA recombinant protein (Position: L21-S95). Human PSCA shares 66% amino acid (aa) sequence identity with mouse PSCA. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PSCA |
Protein | Prostate stem cell antigen |
Uniprot ID | O43653 |
Function | May be involved in the regulation of cell proliferation. Has a cell-proliferation inhibition activity in vitro. |
Tissue Specificity | Highly expressed in prostate (basal, secretory and neuroendocrine epithelium cells). Also found in bladder (transitional epithelium), placenta (trophoblasts), stomach (neuroendocrine cells), colon (neuroendocrine cells) and kidney (collecting ducts). Overexpressed in prostate cancers and expression is correlated with tumor stage, grade and androgen- independence. Highly expressed in prostate cancer bone metastases. Expressed in gastric epithelial cells, mainly in the isthmus (at protein level). Not detected in normal intestinal epithelium (at protein level). |
Sub-cellular localization | Cell membrane; Lipid-anchor, GPI-anchor. |
Sequence Similarities | Contains 1 UPAR/Ly6 domain. |
Aliases | PRO 232 antibody|PRO232 antibody|Prostate stem cell antigen antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- PSCA antibody, ASA-B1589, Western blotting
All lanes: Anti PSCA (ASA-B1589) at 0.5ug/ml
Lane 1: Rat Stomach Tissue Lysate at 50ug
Lane 2: Mouse Stomach Tissue Lysate at 50ug
Predicted bind size: 13KD
Observed bind size: 25KD
All lanes: Anti PSCA (ASA-B1589) at 0.5ug/ml
Lane 1: Rat Stomach Tissue Lysate at 50ug
Lane 2: Mouse Stomach Tissue Lysate at 50ug
Predicted bind size: 13KD
Observed bind size: 25KD
