More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| kallikrein-related peptidase 3 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human PSA recombinant protein (Position: I25-P261). Human PSA shares 54% amino acid (aa) sequence identity with rat PSA. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | KLK3 |
Protein | Prostate-specific antigen |
Uniprot ID | P07288 |
Function | Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the peptidase S1 family. Kallikrein subfamily. |
Aliases | antigen, prostate-specific antibody|APS antibody|Gamma seminoprotein antibody|Gamma-seminoprotein antibody|hK3 antibody|Kallikrein 3 antibody|Kallikrein 3, (prostate specific antigen) antibody|Kallikrein related peptidase 3 antibody|Kallikrein-3 antibody|KLK 3 antibody|KLK2A1 antibody|KLK3 antibody|KLK3_HUMAN antibody|P 30 antigen antibody|P-30 antigen antibody|P30 antigen antibody|Prostate Specific Antigen antibody|Prostate-specific antigen antibody|PSA antibody|Semenogelase antibody|Seminin antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- PSA antibody, ASA-B1588, Western blotting
All lanes: Anti PSA (ASA-B1588) at 0.5ug/ml
WB: Recombinant Human PSA Protein 0.5ng
Predicted bind size: 25KD
Observed bind size: 25KD
All lanes: Anti PSA (ASA-B1588) at 0.5ug/ml
WB: Recombinant Human PSA Protein 0.5ng
Predicted bind size: 25KD
Observed bind size: 25KD
Anti- PSA antibody, ASA-B1588, Western blotting
All lanes: Anti PSA (ASA-B1588) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 29KD
Observed bind size: 29KD
All lanes: Anti PSA (ASA-B1588) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 29KD
Observed bind size: 29KD
