More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| coagulation factor II(thrombin) | Polyclonal | IgG | Rabbit | Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of rat Prothrombin(600-612aa THVFRLKRWMQKV). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | F2 |
Protein | Prothrombin |
Uniprot ID | P18292 |
Function | Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | Belongs to the peptidase S1 family. |
Aliases | coagulation factor II(thrombin) antibody|Coagulation factor II antibody|Coagulation factor II precursor antibody|Coagulation factor II thrombin antibody|Dysprothrombinemia antibody|EC 3.4.21.5 antibody|F 2 antibody|F2 antibody|Factor II antibody|Hyperprothrombinemia antibody|Hypoprothrombinemia antibody|Prepro coagulation factor II antibody|Prothrombin antibody|Prothrombin B chain antibody|prothrombin B-chain antibody|PT antibody|RPRGL2 antibody|Serine protease antibody|THPH1 antibody|THRB antibody|THRB_HUMAN antibody|Thrombin antibody|Thrombin heavy chain antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- F2 antibody, ASA-B1586, Western blotting
All lanes: Anti F2 (ASA-B1586) at 0.5ug/ml
WB : Rat Testis Tissue Lysate at 50ug
Predicted bind size: 70KD
Observed bind size: 100KD
All lanes: Anti F2 (ASA-B1586) at 0.5ug/ml
WB : Rat Testis Tissue Lysate at 50ug
Predicted bind size: 70KD
Observed bind size: 100KD
