More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| proprotein convertase subtilisin/kexin type 4 | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of mouse Proprotein convertase PC4(117-133aa DPWFSKQWYMNKEIQQD), different from the related rat sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PCSK4 |
Protein | Proprotein convertase subtilisin/kexin type 4 |
Uniprot ID | P29121 |
Function | Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. |
Tissue Specificity | Expressed abundantly in the testis. |
Sub-cellular localization | |
Sequence Similarities | Belongs to the peptidase S8 family. Furin subfamily. |
Aliases | PC4 antibody|PCSK4 antibody|PCSK4_HUMAN antibody|Proprotein convertase 4 antibody|Proprotein convertase PC4 antibody|Proprotein convertase subtilisin/kexin type 4 antibody|SPC5 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Proprotein convertase PC4 antibody, ASA-B1581, Western blotting
All lanes: Anti Proprotein convertase PC4 (ASA-B1581) at 0.5ug/ml
WB: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 83KD
Observed bind size: 83KD
All lanes: Anti Proprotein convertase PC4 (ASA-B1581) at 0.5ug/ml
WB: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 83KD
Observed bind size: 83KD
