More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| prolactin | Polyclonal | IgG | Rabbit | Human | ELISA, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Prolactin recombinant protein (Position: L29-227C). Human Prolactinshares60%and 63% aminoacid(aa)sequenceidentitywith mouseandrat Prolactin, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PRL |
Protein | Prolactin |
Uniprot ID | P01236 |
Function | Prolactin acts primarily on the mammary gland by promoting lactation. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the somatotropin/prolactin family. |
Aliases | Decidual prolactin antibody|Lactogenic Hormone antibody|Luteotropic Hormone antibody|Mammotropin antibody|PRL antibody|Prolactin antibody|Prolactin precursor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Prolactin antibody, PB361, Western blotting
All lanes: Anti Prolactin (ASA-B1573) at 0.5ug/ml
WB: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 25KD
Observed bind size: 25KD
All lanes: Anti Prolactin (ASA-B1573) at 0.5ug/ml
WB: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 25KD
Observed bind size: 25KD
