More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| protein phosphatase 1, regulatory subunit 15B | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human PPP1R15B(15-31aa RAGFRFWPPFFPRRSQA). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PPP1R15B |
Protein | Protein phosphatase 1 regulatory subunit 15B |
Uniprot ID | Q5SWA1 |
Function | Maintains low levels of EIF2S1 phosphorylation in unstressed cells by promoting its dephosphorylation by PP1. |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | |
Aliases | CREP antibody|FLJ14744 antibody|PPP1R15B antibody|Protein phosphatase 1, regulatory(inhibitor) subunit 15B antibody|Protein phosphatase 1, regulatory subunit 15B antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PPP1R15B antibody, ASA-B1562, Western blotting
All lanes: Anti PPP1R15B(ASA-B1562) at 0.5ug/ml
WB: A549 Whole Cell Lysate at 40ug
Predicted bind size: 79KD
Observed bind size: 79KD
All lanes: Anti PPP1R15B(ASA-B1562) at 0.5ug/ml
WB: A549 Whole Cell Lysate at 40ug
Predicted bind size: 79KD
Observed bind size: 79KD
