More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| peroxisome proliferator-activated receptor gamma | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human PPAR gamma(45-62aa PHYEDIPFTRTDPVVADY), different from the rat and mouse sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PPARG |
Protein | Peroxisome proliferator-activated receptor gamma(PPAR-gamma) |
Uniprot ID | P37231 |
Function | Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity). |
Tissue Specificity | Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary. |
Sub-cellular localization | Nucleus. Cytoplasm. Note: Redistributed from the nucleus to the cytosol through a MAP2K1/MEK1-dependent manner. CCRN4L/NOC enhances its nuclear translocation. |
Sequence Similarities | Belongs to the nuclear hormone receptor family. NR1 subfamily. |
Aliases | CIMT1 antibody|GLM1 antibody|HUMPPARG antibody|NR1C3 antibody|Nuclear receptor subfamily 1 group C member 3 antibody|OTTHUMP00000185032 antibody|OTTHUMP00000185036 antibody|PAX8/PPARG Fusion Gene antibody|Peroxisome proliferator activated nuclear receptor gamma variant 1 antibody|Peroxisome proliferator activated receptor gamma 1 antibody|Peroxisome Proliferator Activated Receptor gamma antibody|Peroxisome proliferator-activated receptor gamma antibody|PPAR gamma antibody|PPAR-gamma antibody|PPARG antibody|PPARG_HUMAN antibody|PPARG1 antibody|PPARG2 antibody|PPARG3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PPAR gamma antibody, ASA-B1559, Western blotting
Lane 1: MM453 Cell Lysate
Lane 2: MM231 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: HT1080 Cell Lysate
Lane 1: MM453 Cell Lysate
Lane 2: MM231 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: HT1080 Cell Lysate
