More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| paraoxonase 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human PON2(189-203aa FLKYLETYLNLHWAN), identical to the related mouse sequence, and different from the related rat sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PON2 |
Protein | Serum paraoxonase/arylesterase 2 |
Uniprot ID | Q15165 |
Function | Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. |
Tissue Specificity | Widely expressed with highest expression in liver, lung, placenta, testis and heart. |
Sub-cellular localization | Membrane. |
Sequence Similarities | Belongs to the paraoxonase family. |
Aliases | A esterase 2 antibody|A-esterase 2 antibody|Aromatic esterase 2 antibody|Paraoxonase 2 antibody|Paraoxonase nirs antibody|PON 2 antibody|PON2 antibody|PON2_HUMAN antibody|Serum aryldialkylphosphatase 2 antibody|Serum paraoxonase/arylesterase 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PON2 antibody, ASA-B1555, All Western blotting
All lanes: Anti-PON2(ASA-B1555) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Rat Lung Tissue Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 40ug
Lane 4: Rat Testis Tissue Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: HEPA Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Lane 8: JURKAT Whole Cell Lysate at 40ug
Lane 9: SKOV Whole Cell Lysate at 40ug
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All lanes: Anti-PON2(ASA-B1555) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Rat Lung Tissue Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 40ug
Lane 4: Rat Testis Tissue Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: HEPA Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Lane 8: JURKAT Whole Cell Lysate at 40ug
Lane 9: SKOV Whole Cell Lysate at 40ug