More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| phospholipase D1, phosphatidylcholine-specific | Polyclonal | IgG | Rabbit | Human, Mouse | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human PLD1 recombinant protein (Position: M1-H330). Human PLD1 shares 91.8% and 90.9% amino acid (aa) sequence identity with mouse and rat PLD1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PLD1 |
Protein | Phospholipase D1 |
Uniprot ID | Q13393 |
Function | Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic (By similarity). |
Tissue Specificity | Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine. |
Sub-cellular localization | Cytoplasm, perinuclear region . Endoplasmic reticulum membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus membrane ; Lipid-anchor ; Cytoplasmic side . Late endosome membrane ; Lipid- anchor ; Cytoplasmic side . |
Sequence Similarities | Belongs to the phospholipase D family. |
Aliases | Choline phosphatase 1 antibody|hPLD 1 antibody|hPLD1 antibody|Human ARF activated phosphatidylcholine specific phospholipase D1a antibody| Phosphatidylcholine hydrolyzing phospholipase D1 antibody|Phosphatidylcholine -hydrolyzing phospholipase D1 antibody|Phospholipase D1 antibody| Phospholipase D1 phosphatidylcholine specific antibody| PhospholipaseD1 antibody|PL D1 antibody|PLD 1 antibody|PLD1 antibody|PLD1_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PLD1 antibody, ASA-B1541, Western blotting
All lanes: Anti PLD1 (ASA-B1541) at 0.5ug/ml
Lane 1: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 2: 22RV1 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 124KD
Observed bind size: 124KD
All lanes: Anti PLD1 (ASA-B1541) at 0.5ug/ml
Lane 1: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 2: 22RV1 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 124KD
Observed bind size: 124KD
