More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| phospholipase C, beta 1 (phosphoinositide-specific) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human PLCB1 recombinant protein (Position: V888-E1059). Human PLCB1 shares 99% amino acid (aa) sequence identity with both mouse and rat PLCB1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PLCB1 |
Protein | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 |
Uniprot ID | Q9NQ66 |
Function | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. |
Tissue Specificity | |
Sub-cellular localization | Nucleus membrane . Cytoplasm . Note: Colocalizes with the adrenergic receptors, ADREN1A and ADREN1B, at the nuclear membrane of cardiac myocytes. |
Sequence Similarities | Contains 1 C2 domain. |
Aliases | 1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase beta 1 antibody|1-phosphatidyl-D-myo-inositol-4,5-bisphosphate antibody|1-phosphatidylinositol 4 antibody|5-bisphosphate phosphodiesterase beta-1 antibody|EIEE12 antibody|Inositoltrisphosphohydrolase antibody|Monophosphatidylinositol phosphodiesterase antibody|Phosphb antibody|Phosphoinositidase C antibody|Phosphoinositide phospholipase C antibody|Phosphoinositide phospholipase C-beta 1 antibody|Phosphoinositide phospholipase C-beta-1 antibody|Phospholipase C beta 1 (phosphoinositide-specific) antibody|Phospholipase C I antibody|Phospholipase C-beta-1 antibody|Phospholipase C-I antibody|PI PLC antibody|PLC 1 antibody|PLC beta 1 antibody|PLC I antibody|PLC-154 antibody|PLC-beta-1 antibody|PLC-I antibody|PLC154 antibody|Plcb antibody|Plcb1 antibody|Plcb1 protein antibody|PLCB1_HUMAN antibody|PLCbeta1 antibody|Triphosphoinositide phosphodiesterase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- PLCB1 antibody, ASA-B1540, Western blotting
All lanes: Anti PLCB1 (ASA-B1540) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: Mouse Testis Tissue Lysate at 50ug
Lane 4: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 139KD
Observed bind size: 139KD
All lanes: Anti PLCB1 (ASA-B1540) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: Mouse Testis Tissue Lysate at 50ug
Lane 4: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 139KD
Observed bind size: 139KD
