More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| phosphoinositide-3-kinase, regulatory subunit 1(alpha) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human PI 3 Kinase p85 alpha(65-80aa ERGDFPGTYVEYIGRK), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PIK3R1 |
Protein | Phosphatidylinositol 3-kinase regulatory subunit alpha(PI3-kinase regulatory subunit alpha/PI3K regulatory subunit alpha/PtdIns-3-kinase regulatory subunit alpha) |
Uniprot ID | P27986 |
Function | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923). |
Tissue Specificity | Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level). |
Sub-cellular localization | |
Sequence Similarities | Belongs to the PI3K p85 subunit family. |
Aliases | GRB 1 antibody|GRB1 antibody|p85 alpha antibody|p85 antibody|p85a antibody|P85A_HUMAN antibody|Phosphatidylinositol 3 kinase associated p 85 alpha antibody|Phosphatidylinositol 3 kinase associated p85 alpha antibody|Phosphatidylinositol 3 kinase regulatory 1 antibody|Phosphatidylinositol 3 kinase regulatory alpha subunit antibody|Phosphatidylinositol 3 kinase regulatory subunit alpha antibody|Phosphatidylinositol 3 kinase regulatory subunit polypeptide 1 antibody|Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1(p85 alpha) antibody|Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody|Phosphatidylinositol 3-kinase regulatory subunit alpha antibody|Phosphoinositide 3 kinase regulatory subunit 1 antibody|Phosphoinositide 3 kinase regulatory subunit polypeptide 1(p85 alpha) antibody|Phosphoinositide 3 kinase regulatory subunit polypeptide 1 antibody|Phosphoinositide 3 kinase, regulatory subunit 1(alpha) antibody|PI3 kinase p85 alpha subunit antibody|PI3 kinase p85 subunit alpha antibody|PI3-kinase regulatory subunit alpha antibody|PI3-kinase subunit p85-alpha antibody|PI3K antibody|PI3K regulatory subunit alpha antibody|PIK3R 1 antibody|PIK3R1 antibody|PtdIns 3 kinase p85 alpha antibody|PtdIns-3-kinase regulatory subunit alpha antibody|PtdIns-3-kinase regulatory subunit p85-alpha antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PI 3 Kinase p85 alpha antibody, ASA-B1519, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: COLO Cell Lysate
Lane 4: SW620 Cell Lysate
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: COLO Cell Lysate
Lane 4: SW620 Cell Lysate
