More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
peroxiredoxin 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
A synthetic peptide corresponding to a sequence at the C-terminus of human Peroxiredoxin 2(181-198aa DTIKPNVDDSKEYFSKHN), identical to the related mouse and rat sequences. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PRDX2 |
Protein | Peroxiredoxin-2 |
Uniprot ID | P32119 |
Function | Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the AhpC/TSA family. |
Aliases | MGC4104 antibody|Natural killer cell enhancing factor B antibody|Natural killer cell-enhancing factor B antibody|Natural Killer Enhancing Factor B antibody|NKEF B antibody|NKEF-B antibody|NKEFB antibody|Peroxiredoxin-2 antibody|PRDX 2 antibody|PRDX2 antibody|PRDX2_HUMAN antibody|PrP antibody|PRX2 antibody|PRXII antibody|TDPX1 antibody|Thiol Specific Antioxidant 1 antibody|Thiol specific antioxidant protein antibody|Thiol-specific antioxidant protein antibody|Thioredoxin Dependent Peroxide Reductase 1 antibody|Thioredoxin peroxidase 1 antibody|Thioredoxin-dependent peroxide reductase 1 antibody|Torin antibody|TPX1 antibody|TSA antibody |
Application Details
Application | Concentration* | Species | Validated Using** |
Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Peroxiredoxin 2 antibody, ASA-B1505, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: 293T Cell Lysate
Lane 6: A549 Cell Lysate