More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| eukaryotic translation initiation factor 2-alpha kinase 3 | Polyclonal | IgG | Rabbit | Human | ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human PERK(163-176aa QWDQDRESMETVPF). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | EIF2AK3 |
Protein | Eukaryotic translation initiation factor 2-alpha kinase 3 |
Uniprot ID | Q9NZJ5 |
Function | Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function (By similarity). |
Tissue Specificity | Ubiquitous. A high level expression is seen in secretory tissues. |
Sub-cellular localization | Endoplasmic reticulum membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. |
Aliases | DKFZp781H1925 antibody|E2AK3_HUMAN antibody|EC 2.7.11.1 antibody|EIF2AK3 antibody|Eukaryotic translation initiation factor 2 alpha kinase 3 antibody|Eukaryotic translation initiation factor 2-alpha kinase 3 antibody|Heme regulated EIF2 alpha kinase antibody|HRI antibody|HsPEK antibody|Pancreatic eIF2 alpha kinase antibody|Pancreatic eIF2-alpha kinase antibody|PEK antibody|PRKR like endoplasmic reticulum kinase antibody|PRKR-like endoplasmic reticulum kinase antibody|WRS antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PERK antibody, ASA-B1502, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human EIF2AK3, 35.1KD(162aa tag+ D93-R246)
Lane 1: Recombinant Human EIF2AK3 Protein 10ng
Lane 2: Recombinant Human EIF2AK3 Protein 5ng
Lane 3: Recombinant Human EIF2AK3 Protein 2.5ng
Recombinant Protein Detection Source: E.coli derived -recombinant Human EIF2AK3, 35.1KD(162aa tag+ D93-R246)
Lane 1: Recombinant Human EIF2AK3 Protein 10ng
Lane 2: Recombinant Human EIF2AK3 Protein 5ng
Lane 3: Recombinant Human EIF2AK3 Protein 2.5ng
Anti-PERK antibody, ASA-B1502, ICC
ICC: A549 Cell
ICC: A549 Cell
