More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| periostin, osteoblast specific factor | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of mouse Periostin(24-41aa NSYYDKVLAHSRIRGRDQ), identical to the related rat sequence, and different from the related human sequence by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | POSTN |
Protein | Periostin |
Uniprot ID | Q62009 |
Function | Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX. |
Tissue Specificity | Preferentially expressed in periosteum and periodontal ligament. Also expressed in the developing and adult heart. |
Sub-cellular localization | Golgi apparatus. Secreted, extracellular space, extracellular matrix. Note: Colocalizes with BMP1 in the Golgi. |
Sequence Similarities | Contains 1 EMI domain. |
Aliases | MGC119510 antibody|MGC119511 antibody|OSF 2 antibody|OSF-2 antibody|OSF2 antibody|Osteoblast specific factor 2(fasciclin I like) antibody|osteoblast specific factor 2(fasciclin I-like) antibody|Osteoblast specific factor 2 antibody|Osteoblast specific factor antibody|Osteoblast-specific factor 2 antibody|PDLPOSTN antibody|Periodontal ligament specific periostin antibody|Periostin antibody|Periostin isoform thy2 antibody|Periostin isoform thy4 antibody|Periostin isoform thy6 antibody|Periostin isoform thy8 antibody|Periostin osteoblast specific factor antibody|PN antibody|POSTN antibody|POSTN_HUMAN antibody|RP11 412K4.1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Periostin antibody, ASA-B1501, Western blotting
All lanes: Anti Periostin (ASA-B1501) at 0.5ug/ml
WB: Mouse Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 93KD
Observed bind size: 93KD
All lanes: Anti Periostin (ASA-B1501) at 0.5ug/ml
WB: Mouse Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 93KD
Observed bind size: 93KD
