PD-L1 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
CD274 molecule	Polyclonal	IgG	Rabbit	Human	WB	Immunogen affinity purified.
Immunogen
E.coli-derived human PD-L1 recombinant protein (Position: E45-T290). Human PD-L1 shares 69% amino acid (aa) sequence identity with mouse PD-L1.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	CD274
Protein	Programmed cell death 1 ligand 1
Uniprot ID	Q9NZQ7
Function	Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Tissue Specificity	Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Sub-cellular localization	Isoform 1: Cell membrane; Single-pass type I membrane protein.
Sequence Similarities	Belongs to the immunoglobulin superfamily. BTN/MOG family.
Aliases	B7 H antibody|B7 H1 antibody|B7 homolog 1 antibody|B7-H1 antibody|B7H antibody|B7H1 antibody|CD 274 antibody|CD274 antibody|Cd274 antibody|CD274 antigen antibody|CD274 molecule antibody|MGC142294 antibody|MGC142296 antibody|OTTHUMP00000021029 antibody|PD L1 antibody|PD-L1 antibody|PD1L1_HUMAN antibody|PDCD1 ligand 1 antibody|PDCD1L1 antibody|PDCD1LG1 antibody|PDL 1 antibody|PDL1 antibody|Programmed cell death 1 ligand 1 antibody|Programmed death ligand 1 antibody|RGD1566211 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolutio's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information