More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| platelet-derived growth factor beta polypeptide | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human PDGF beta recombinant protein (Position: S82-T190). Human PDGF beta shares 89% amino acid (aa) sequence identity with both mouse and rat PDGF beta. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PDGFB |
Protein | Platelet-derived growth factor subunit B |
Uniprot ID | P01127 |
Function | Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity). |
Tissue Specificity | Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung. |
Sub-cellular localization | Secreted. Note: Released by platelets upon wounding. |
Sequence Similarities | Belongs to the PDGF/VEGF growth factor family. |
Aliases | Becaplermin antibody|c sis antibody|FLJ12858 antibody|Oncogene SIS antibody|PDGF 2 antibody|PDGF B antibody|PDGF B chain antibody|PDGF subunit B antibody|PDGF-2 antibody|PDGF2 antibody|Pdgfb antibody|PDGFB_HUMAN antibody|Platelet derived growth factor 2 antibody|Platelet derived growth factor B chain antibody|Platelet derived growth factor beta antibody|Platelet derived growth factor beta polypeptide (oncogene SIS) antibody|Platelet derived growth factor beta polypeptide (simian sarcoma viral (v sis) oncogene homolog) antibody|Platelet derived growth factor beta polypeptide antibody|Platelet-derived growth factor B chain antibody|Platelet-derived growth factor beta polypeptide antibody|Platelet-derived growth factor subunit B antibody|Proto-oncogene c-Sis antibody|Simian sarcoma viral (v sis) oncogene homolog antibody|SIS antibody|SSV antibody|v sis platelet derived growth factor beta polypeptide antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- PDGF beta antibody, ASA-B1494, Western blotting
All lanes: Anti PDGF beta (ASA-B1494) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 13KD
All lanes: Anti PDGF beta (ASA-B1494) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 13KD
