More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| programmed cell death 4(neoplastic transformation inhibitor) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human PDCD4(351-368aa LKELEVPHFHHELVYEAI), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PDCD4 |
Protein | Programmed cell death protein 4 |
Uniprot ID | Q53EL6 |
Function | Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity). |
Tissue Specificity | Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma. |
Sub-cellular localization | Nucleus. Cytoplasm. Note: Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum. |
Sequence Similarities | Belongs to the PDCD4 family. |
Aliases | Death up-regulated gene protein antibody|Dug antibody|H731 antibody|Ma3 antibody|MGC33046 antibody|MGC33047 antibody|Neoplastic transformation inhibitor antibody|Neoplastic transformation inhibitor protein antibody|Nuclear antigen H731 antibody|Nuclear antigen H731 like antibody|Nuclear antigen H731 like protein antibody|Nuclear antigen H731-like antibody|OTTHUMP00000020483 antibody|PDCD 4 antibody|Pdcd4 antibody|PDCD4_HUMAN antibody|Programmed cell death 4 antibody|programmed cell death 4(neoplastic transformation inhibitor) antibody|Programmed cell death protein 4 antibody|Protein 197/15a antibody|Protein MA-3 antibody|RP11 348N5.4 antibody|Tis antibody|Topoisomerase-inhibitor suppressed protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PDCD4 antibody, ASA-B1493, All Western blotting
All lanes: Anti-PDCD4(ASA-B1493) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: HL60 Whole Cell Lysate at 40ug
Predicted bind size: 52KD
Observed bind size: 52KD
All lanes: Anti-PDCD4(ASA-B1493) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: HL60 Whole Cell Lysate at 40ug
Predicted bind size: 52KD
Observed bind size: 52KD
