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Paxillin Antibody (monoclonal) View larger

Paxillin Antibody (monoclonal)

ASA-B1481

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
paxillin Monoclonal IgG Mouse Chicken, Human, Mouse, Rat ICC, WB Ascites

Immunogen

C-terminal part of recombinant chicken paxillin(amino acids 305-559).

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.

Concentration

 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 PXN

Protein

 Paxillin

Uniprot ID

 Human(P49023), Mouse(Q8VI36), Rat(Q66H76)

Function

 Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

Tissue Specificity

Sub-cellular localization

 Cytoplasm, cytoskeleton.

Sequence Similarities

 Belongs to the paxillin family.

Aliases

Application Details

Application Concentration* Species Validated Using**
Western blot1-2μg/mlHuman, mouse, rat, chickenAssaySolutio's ECL kit
ImmunocytochemistrySuitableHuman, mouse, rat, chickenAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Paxillin antibody (monoclonal), ASA-B1481, Western blotting
Lane 1: 293T Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: MM231 Cell Lysate
Lane 5: JURKAT Cell Lysate

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