More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| patched 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Patched recombinant protein (Position: D1219-N1447). Human Patched shares 91% amino acid (aa) sequence identity with mouse Patched. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PTCH1 |
Protein | Protein patched homolog 1 |
Uniprot ID | Q13635 |
Function | Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. |
Tissue Specificity | In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin. |
Sub-cellular localization | Membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the patched family. |
Aliases | A230106A15Rik antibody|BCNS antibody|FLJ26746 antibody|FLJ42602 antibody|Holoprosencephaly 7 antibody|HPE7 antibody|mes antibody|NBCCS antibody|OTTHUMP00000021709 antibody|OTTHUMP00000021710 antibody|Patched antibody|Patched (Drosophila) homolog antibody|Patched 1 antibody|Patched homolog (Drosophila) antibody|Patched homolog 1 (Drosophila) antibody|Patched homolog 1 antibody|Patched protein homolog 1 antibody|Protein patched homolog 1 antibody|PTC antibody|PTC1 antibody|PTC1_HUMAN antibody|PTCH antibody|PTCH protein +12b antibody|PTCH protein +4' antibody|PTCH protein -10 antibody|PTCH protein antibody|PTCH1 antibody|PTCH1 protein antibody|PTCH11 antibody|Ptch2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Patched antibody, ASA-B1480, Western blotting
All lanes: Anti Patched (ASA-B1480) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Lane 4: Mouse Liver Tissue Lysate at 50ug
Predicted bind size: 160KD
Observed bind size: 160KD
All lanes: Anti Patched (ASA-B1480) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Lane 4: Mouse Liver Tissue Lysate at 50ug
Predicted bind size: 160KD
Observed bind size: 160KD
