More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| p21 protein(Cdc42/Rac)-activated kinase 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human PAK1(1-14aa MSNNGLDIQDKPPA), different from the related mouse and rat sequences by on amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PAK1 |
Protein | Serine/threonine-protein kinase PAK 1 |
Uniprot ID | Q13153 |
Function | Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at 'Ser-338' and 'Ser-339' resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2. Phosphorylates SNAI1 at 'Ser-246' promoting its transcriptional repressor activity by increasing its accumulation in the nucleus. In podocytes, promotes NR3C2 nuclear localization. Required for atypical chemokine receptor ACKR2- induced phosphorylation of LIMK1 and cofilin (CFL1) and for the up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F- actin cluster formation performed by SHANK3, maybe through CFL1 phosphorylation and inactivation. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. Cell junction, focal adhesion. Cell membrane. Cell projection, ruffle membrane. Note: Recruited to the cell membrane by interaction with CDC42 and RAC1. Recruited to focal adhesions upon activation. Colocalized with CIB1 within membrane ruffles during cell spreading upon readhesion to fibronectin. |
Sequence Similarities | Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. |
Aliases | Alpha PAK antibody|Alpha-PAK antibody|MGC130000 antibody|MGC130001 antibody|p21 activated kinase 1 antibody|p21 protein(Cdc42/Rac) activated kinase 1 antibody|p21-activated kinase 1 antibody|p21/Cdc42/Rac1 activated kinase 1(yeast Ste20 related) antibody|p21/Cdc42/Rac1 activated kinase 1 antibody|p21/Cdc42/Rac1-activated kinase 1(STE20 homolog, yeast) antibody|p65 PAK antibody|p65-PAK antibody|PAK alpha antibody|PAK-1 antibody|Pak1 antibody|PAK1_HUMAN antibody|Paka antibody|PAKalpha antibody|Protein kinase MUK2 antibody|Rac/p21-activated kinase antibody|Serine/threonine-protein kinase PAK 1 antibody|STE20 homolog(yeast) antibody|STE20 homolog yeast antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-PAK1 antibody, ASA-B1470, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: SW620 Cell Lysate
Lane 4: MCF-7 Cell Lysate
Lane 5: CEM Cell Lysate
Lane 6: HT1080 Cell Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: SW620 Cell Lysate
Lane 4: MCF-7 Cell Lysate
Lane 5: CEM Cell Lysate
Lane 6: HT1080 Cell Lysate
