More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| nerve growth factor receptor | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human p75 NGF Receptor(397-414aa TLDALLAALRRIQRADLV), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NGFR |
Protein | Tumor necrosis factor receptor superfamily member 16 |
Uniprot ID | P08138 |
Function | Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. |
Tissue Specificity | |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Contains 1 death domain. |
Aliases | CD271 antibody|CD271 antigen antibody|Gp80 LNGFR antibody|Gp80-LNGFR antibody|Low affinity nerve growth factor receptor antibody|Low affinity neurotrophin receptor p75NTR antibody|Low affinity neurotrophin receptor p75NTR antibody|Low-affinity nerve growth factor receptor antibody|Nerve growth factor receptor antibody|Nerve growth factor receptor TNFR superfamily member 16 antibody|NGF receptor antibody|NGF receptor antibody|Ngfr antibody|p75 ICD antibody|p75 ICD antibody|p75 Neurotrophin receptor antibody|p75 NTR antibody|p75(NTR) antibody|p75NTR antibody|TNFR Superfamily Member 16 antibody|TNFRSF16 antibody|TNR16_HUMAN antibody|Tumor necrosis factor receptor superfamily member 16 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-p75 NGF Receptor antibody, ASA-B1467, All Western blotting
All lanes: Anti-NGFR(ASA-B1467) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: SHG Whole Cell Lysate at 40ug
Lane 3: NEURO Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 45KD
All lanes: Anti-NGFR(ASA-B1467) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: SHG Whole Cell Lysate at 40ug
Lane 3: NEURO Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 45KD
