More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cyclin-dependent kinase inhibitor 1C(p57, Kip2) | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of mouse p57 Kip2(51-70aa RLAELNAEDQNRWDFNFQQD), identical to the related rat sequence, and different from the related human sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CDKN1C |
Protein | Cyclin-dependent kinase inhibitor 1C |
Uniprot ID | P49919 |
Function | Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non- proliferative state throughout life. |
Tissue Specificity | Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. High levels are seen in the placenta while low levels are seen in the liver. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | |
Aliases | Beckwith Wiedemann syndrome antibody|BWCR antibody|BWS antibody|CDKI antibody|CDKN 1C antibody|CDKN1C antibody|CDN1C_HUMAN antibody|Cyclin dependent kinase inhibitor 1C antibody|Cyclin dependent kinase inhibitor p57 antibody|Cyclin-dependent kinase inhibitor 1C antibody|Cyclin-dependent kinase inhibitor p57 antibody|KIP 2 antibody|KIP2 antibody|p57 antibody|p57 Kip 2 antibody|p57Kip2 antibody|WBS antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-p57 Kip2 antibody, ASA-B1463, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
