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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| ribonucleotide reductase M2 B(TP53 inducible) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human p53R2(318-332aa EGKTNFFEKRVSEYQ), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | RRM2B |
Protein | Ribonucleoside-diphosphate reductase subunit M2 B |
Uniprot ID | Q7LG56 |
Function | Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. |
Tissue Specificity | Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma. |
Sub-cellular localization | Cytoplasm. Nucleus. Note: Translocates from cytoplasm to nucleus in response to DNA damage. |
Sequence Similarities | Belongs to the ribonucleoside diphosphate reductase small chain family. |
Aliases | DKFZp686M05248 antibody|MGC102856 antibody|MGC42116 antibody|MTDPS8A antibody|MTDPS8B antibody|p53 inducible ribonucleotide reductase small subunit 2 homolog antibody|p53 inducible ribonucleotide reductase small subunit 2 like protein antibody|p53 R2 antibody|p53-inducible ribonucleotide reductase small subunit 2-like protein antibody|p53R2 antibody|Ribonucleoside diphosphate reductase M2 subunit B antibody|Ribonucleoside-diphosphate reductase subunit M2 B antibody|Ribonucleotide reductase M2 B(TP53 inducible) antibody|Ribonucleotide reductase M2 B antibody|Ribonucleotide reductase small subunit like 2 p53 inducible antibody|RIR2B_HUMAN antibody|RRM 2B antibody|RRM2B antibody|TP53 inducible ribonucleotide reductase M2 B antibody|TP53-inducible ribonucleotide reductase M2 B antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-p53R2 antibody, ASA-B1462, Western blotting
Lane 1: Rat Thymus Tissue Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: A431 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 1: Rat Thymus Tissue Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: A431 Cell Lysate
Lane 4: HELA Cell Lysate
