More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| purinergic receptor P2X, ligand-gated ion channel, 7 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
| A synthetic peptide corresponding to a sequence at the N-terminus of rat P2RX7(1-20aa MPACCSWNDVFQYETNKVTR), different from the related mouse sequence by one amino acid, and from the related human sequence by two amino acids. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | P2RX7 |
Protein | P2X purinoceptor 7 |
Uniprot ID | Q64663 |
Function | Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | |
Aliases | ATP receptor antibody|P2X purinoceptor 7 antibody|P2X7 antibody|P2Z receptor antibody|Purinergic receptor antibody|purinergic receptor P2X, ligand gated ion channel, 7 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-P2RX7 antibody, ASA-B1455, Western blotting
WB: U87 Cell Lysate
