More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| ATP-binding cassette, sub-family A(ABC1), member 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human P180 Lamellar Body Protein(271-288aa FTYTALTIARAVVQEKER), different from the related mouse sequence by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ABCA3 |
Protein | ATP-binding cassette sub-family A member 3 |
Uniprot ID | Q99758 |
Function | Plays an important role in the formation of pulmonary surfactant, probably by transporting lipids such as cholesterol. |
Tissue Specificity | Highly expressed in lung, followed by brain, pancreas, skeletal muscle and heart. Weakly expressed in placenta, kidney and liver. Also expressed in medullary thyroid carcinoma cells (MTC) and in C-cell carcinoma. |
Sub-cellular localization | Membrane ; Multi-pass membrane protein . |
Sequence Similarities | Belongs to the ABC transporter superfamily. ABCA family. |
Aliases | ABC 3 antibody|ABC C antibody|ABC C transporter antibody|ABC transporter 3 antibody|ABC-C transporter antibody|ABC3 antibody|ABCA 3 antibody|Abca3 antibody|ABCA3 protein antibody|ABCC antibody|ATP binding cassette 3 antibody|ATP binding cassette sub family A(ABC1) member 3 antibody|ATP binding cassette sub family A member 3 antibody|ATP binding cassette transporter 3 antibody|ATP-binding cassette 3 antibody|ATP-binding cassette sub-family A member 3 antibody|ATP-binding cassette transporter 3 antibody|CED7. C. elegans, homolog of antibody|EST111653 antibody|LBM 180 antibody|LBM180 antibody|MGC72201 antibody|SMDP3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-P180 Lamellar Body Protein antibody, ASA-B1449, All Western blotting
All lanes: Anti-ABCA3(ASA-B1449) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 169KD
Observed bind size: 191KD
All lanes: Anti-ABCA3(ASA-B1449) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 169KD
Observed bind size: 191KD
