More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| tumor necrosis factor receptor superfamily, member 11b | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Osteoprotegerin(369-389aa IRFLHSFTMYKLYQKLFLEMI), different from the related rat sequence by one amino acid, and different from the related mouse sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TNFRSF11B |
Protein | Tumor necrosis factor receptor superfamily member 11B |
Uniprot ID | O00300 |
Function | Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis. |
Tissue Specificity | Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Contains 2 death domains. |
Aliases | MGC29565 antibody|OCIF antibody|OPG antibody|Osteoclastogenesis inhibitory factor antibody|Osteoprotegerin antibody|TNFRSF 11B antibody|TNFRSF11B antibody|TR 1 antibody|TR1 antibody|TR11B_HUMAN antibody|Tumor necrosis factor receptor superfamily member 11B antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Osteoprotegerin antibody, ASA-B1441, Western blotting
All lanes: Anti Osteoprotegerin (ASA-B1441) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: PC-12 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: M453 Whole Cell Lysate at 40ug
Predicted bind size: 46KD
Observed bind size: 60KD
All lanes: Anti Osteoprotegerin (ASA-B1441) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: PC-12 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: M453 Whole Cell Lysate at 40ug
Predicted bind size: 46KD
Observed bind size: 60KD
