More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| oxysterol binding protein | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human OSBP1(290-304aa WQKSLQYERDQRIRL), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | OSBP |
Protein | Oxysterol-binding protein 1 |
Uniprot ID | P22059 |
Function | Binds cholesterol and a range of oxysterols including 25-hydroxycholesterol (PubMed:15746430, PubMed:17428193). Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes its disassembly (PubMed:15746430). Regulates cholesterol efflux by decreasing ABCA1 stability (PubMed:18450749). |
Tissue Specificity | Widely expressed. |
Sub-cellular localization | Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Note: When bound to oxysterols, translocates to the periphery of Golgi membranes. |
Sequence Similarities | Belongs to the OSBP family. |
Aliases | OSBP 1 antibody|OSBP antibody|OSBP1_HUMAN antibody|Oxysterol binding protein 1 antibody|Oxysterol-binding protein 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-OSBP1 antibody, ASA-B1436, Western blotting
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat Lung Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: A549 Cell Lysate
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat Lung Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: A549 Cell Lysate
