More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| numb homolog (Drosophila) | Polyclonal | IgG | Rabbit | Human, Mouse | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human NUMB(194-220aa SFRVTTATEQAEREEIMKQMQDAKKAE), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NUMB |
Protein | Protein numb homolog |
Uniprot ID | P49757 |
Function | Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. |
Tissue Specificity | |
Sub-cellular localization | Membrane; Peripheral membrane protein. |
Sequence Similarities | Contains 1 PID domain. |
Aliases | c14_5527 antibody|C14orf41 antibody|FLJ31314 antibody|h Numb antibody|h-Numb antibody|Numb antibody|Numb homolog (Drosophila) antibody|Numb homolog antibody|Numb protein homolog antibody|NUMB_HUMAN antibody|Protein numb homolog antibody|Protein S171 antibody|S171 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- NUMB antibody, ASA-B1424, Western blotting
All lanes: Anti NUMB (ASA-B1424) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: NEURO Whole Cell Lysate at 40ug
Predicted bind size: 71KD
Observed bind size: 71KD
All lanes: Anti NUMB (ASA-B1424) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: NEURO Whole Cell Lysate at 40ug
Predicted bind size: 71KD
Observed bind size: 71KD
