More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| nuclear receptor subfamily 5, group A, member 2 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human NR5A2 recombinant protein (Position: K44-R237). Human NR5A2 shares 95% amino acid (aa) sequence identity with both mouse and rat NR5A2. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NR5A2 |
Protein | Nuclear receptor subfamily 5 group A member 2 |
Uniprot ID | O00482 |
Function | Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. |
Tissue Specificity | Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells. |
Sub-cellular localization | Nucleus . |
Sequence Similarities | Belongs to the nuclear hormone receptor family. NR5 subfamily. |
Aliases | Alpha-1-fetoprotein transcription factor antibody|B1-binding factor antibody|B1F antibody|B1F2 antibody|CPF antibody|CYP7A promoter-binding factor antibody|FTF antibody|FTZ F1 antibody|FTZ F1beta antibody|FTZ-F1 antibody|FTZ-F1beta antibody|hB1F 2 antibody|HB1F antibody|hB1F-2 antibody|Hepatocytic transcription factor antibody|Liver receptor homolog 1 antibody|LRH-1 antibody|LRH1 antibody|Nr5a2 antibody|NR5A2_HUMAN antibody|Nuclear receptor subfamily 5 group A member 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- NR5A2 antibody, ASA-B1411, Western blotting
All lanes: Anti NR5A2 (ASA-B1411) at 0.5ug/ml
Lane 1: PANC Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Predicted bind size: 61KD
Observed bind size: 61KD
All lanes: Anti NR5A2 (ASA-B1411) at 0.5ug/ml
Lane 1: PANC Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Predicted bind size: 61KD
Observed bind size: 61KD
