More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| notch 1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Notch1(2487-2506aa QHSYSSPVDNTPSHQLQVPE). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NOTCH1 |
Protein | Neurogenic locus notch homolog protein 1(Notch 1/hN1) |
Uniprot ID | P46531 |
Function | Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO). |
Tissue Specificity | In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues. |
Sub-cellular localization | Cell membrane ; Single-pass type I membrane protein . |
Sequence Similarities | Belongs to the NOTCH family. |
Aliases | 9930111A19RIK antibody|hN1 antibody|Lin-12 antibody|LIN12 antibody|MIS6 antibody|Motch A antibody|mT14 antibody|Neurogenic locus notch homolog protein 1 antibody|Neurogenic locus notch protein homolog antibody|NICD antibody|NOTC1_HUMAN antibody| Notch 1 antibody|Notch 1 extracellular truncation antibody|Notch 1 intracellular domain antibody|NOTCH antibody|Notch gene homolog 1(Drosophila) antibody|Notch homolog 1 translocation associated(Drosophila) antibody|Notch homolog 1, translocation-associated(Drosophila) antibody|NOTCH, Drosophila, homolog of, 1 antibody|notch1 antibody|p300 antibody|TAN 1 antibody|TAN1 antibody|TAN1 antibody|Translocation Associated Notch Homolog antibody|Translocation associated notch protein TAN 1 antibody|Translocation-associated notch protein TAN-1 antibody|xotch antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Notch1 antibody, ASA-B1398, Western blotting
All lanes: Anti Notch1 (ASA-B1398) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 272KD
Observed bind size: 272KD
All lanes: Anti Notch1 (ASA-B1398) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 272KD
Observed bind size: 272KD
