More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| glutamate receptor, ionotropic, N-methyl D-aspartate 2A | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human NMDAR2A recombinant protein (Position: D958-R1300). Human NMDAR2A shares 89% and 90% amino acid (aa) sequence identity with mouse and rat NMDAR2A, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GRIN2A |
Protein | Glutamate receptor ionotropic, NMDA 2A |
Uniprot ID | Q12879 |
Function | NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. |
Sequence Similarities | |
Aliases | Glutamate [NMDA] receptor subunit epsilon 1 antibody|Glutamate [NMDA] receptor subunit epsilon-1 antibody|Glutamate receptor antibody|Glutamate receptor ionotropic N methyl D aspartate 2A antibody|GRIN 2A antibody|GRIN2A antibody|HNR2A antibody|N methyl D aspartate receptor channel, subunit epsilon 1 antibody|N Methyl D Aspartate Receptor Subtype 2A antibody|N methyl D aspartate receptor subunit 2A antibody|N-methyl D-aspartate receptor subtype 2A antibody|NMDA receptor subtype 2A antibody|NMDA Receptor Type 2A antibody|NMDAR 2A antibody|NMDAR2A antibody|NMDE1_HUMAN antibody|NR 2A antibody|NR2A antibody|OTTHUMP00000160135 antibody|OTTHUMP00000174531 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- NMDAR2A antibody, ASA-B1388, Western blotting
All lanes: Anti NMDAR2A (ASA-B1388) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 165KD
Observed bind size: 165KD
All lanes: Anti NMDAR2A (ASA-B1388) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 165KD
Observed bind size: 165KD
