More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human NFAT2(700-716aa AIFLTVSREHERVGCFF). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NFATC1 |
Protein | Nuclear factor of activated T-cells cytoplasmic,1 |
Uniprot ID | O95644 |
Function | Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells. |
Tissue Specificity | Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T- cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T- cells after second antigen exposure. Isoforms IA are widely expressed but not detected in liver nor pancreas, neural expression is strongest in corpus callosum. Isoforms IB are expressed mostly in muscle, cerebellum, placenta and thymus, neural expression in fetal and adult brain, strongest in corpus callosum. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | |
Aliases | cytoplasmic 1 antibody|MGC138448 antibody|NAFATC antibody|NF ATc antibody|NF ATc1 antibody|NF-ATc antibody|NF-ATc1 antibody|NFAC1_HUMAN antibody|NFAT 2 antibody|NFAT transcription complex cytosolic component antibody|NFAT2 antibody|NFATC 1 antibody|NFATc antibody|NFATC1 antibody|Nuclear factor of activated T cells cytoplasmic 1 antibody|Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1 antibody|Nuclear factor of activated T cells cytosolic component 1 antibody|Nuclear factor of activated T-cells antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NFAT2 antibody, ASA-B1371, Western blotting
All lanes: Anti NFAT2 (ASA-B1371) at 0.5ug/ml
Lane 1: PANC Whole Cell Lysate at 40ug
Lane 2: U20S Whole Cell Lysate at 40ug
Predicted bind size: 101KD
Observed bind size: 85KD
All lanes: Anti NFAT2 (ASA-B1371) at 0.5ug/ml
Lane 1: PANC Whole Cell Lysate at 40ug
Lane 2: U20S Whole Cell Lysate at 40ug
Predicted bind size: 101KD
Observed bind size: 85KD
