More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| tachykinin receptor 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Neurokinin 1 Receptor(231-245aa DSSDRYHEQVSAKRK), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TACR1 |
Protein | Substance-P receptor(SPR) |
Uniprot ID | P25103 |
Function | This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the G-protein coupled receptor 1 family. |
Aliases | neurokinin 1 antibody|NK 1 receptor antibody|NK 1R antibody|NK-1 receptor antibody|NK-1R antibody|NK1 receptor antibody|NK1R antibody|NK1R_HUMAN antibody|NKIR antibody|SPR antibody|Substance P receptor antibody|Substance-P receptor antibody|TAC 1R antibody|TAC1R antibody|Tachykinin 1 receptor(substance P receptor neurokinin 1 receptor) antibody|Tachykinin receptor 1 antibody|Tachykinin receptor 1(substance P receptor neurokinin 1 receptor) antibody|TACR1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Neurokinin 1 Receptor antibody, ASA-B1359, Western blotting
All lanes: Anti Neurokinin 1 Receptor (ASA-B1359) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: SCG Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 55KD
All lanes: Anti Neurokinin 1 Receptor (ASA-B1359) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: SCG Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 55KD
