More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| netrin 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Netrin 1(32-51aa AGQAAQPDPCSDENGHPRRC), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NTN1 |
Protein | Netrin-1 |
Uniprot ID | O95631 |
Function | Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis. |
Tissue Specificity | Widely expressed in normal adult tissues with highest levels in heart, small intestine, colon, liver and prostate. Reduced expression in brain tumors and neuroblastomas. Expressed in epididymis (at protein level). |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix . |
Sequence Similarities | Contains 3 laminin EGF-like domains. |
Aliases | NET1_HUMAN antibody|Netrin 1, mouse, homolog of antibody|Netrin 1-like antibody|Netrin-1 antibody|Netrin1 antibody|Ntn 1 antibody|NTN 1L antibody|Ntn1 antibody|NTN1L antibody|Unc6 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Netrin 1 antibody, ASA-B1358, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 1: U87 Cell Lysate
Lane 2: COLO320 Cell Lysate
