More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| nebulin | Monoclonal | IgG | Mouse | Chicken | WB | Ascites |
Immunogen |
| Chicken breast nebulin. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NEB |
Protein | Nebulin |
Uniprot ID | Human(P20929) |
Function | This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin. |
Tissue Specificity | Muscle specific. Located in the thin filament of striated muscle. |
Sub-cellular localization | Cytoplasm, myofibril, sarcomere. Cytoplasm, cytoskeleton. |
Sequence Similarities | Contains 178 nebulin repeats. |
Aliases | NEM2|NEB177D |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 1-2 μg/ml | Chicken | AssaySolutio's ECL kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
