More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| NDRG family member 2 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human NDRG2(212-230aa ELIQKYRNIITHAPNLDNI). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NDRG2 |
Protein | Protein NDRG2 |
Uniprot ID | Q9UN36 |
Function | Contributes to the regulation of the Wnt signaling pathway. Down-regulates CTNNB1-mediated transcriptional activation of target genes, such as CCND1, and may thereby act as tumor suppressor. May be involved in dendritic cell and neuron differentiation. |
Tissue Specificity | Highly expressed in brain, heart, skeletal muscle and salivary gland, and moderately in kidney and liver. Expressed in dendritic cells, but not in other blood cells. Expression levels are low in pancreatic and liver cancer tissues; absent in meningioma. Expressed in low-grade gliomas but present at low levels in glioblastoma. Isoform 1 and isoform 2 are present in brain neurons and up-regulated in Alzheimer disease (at protein level). |
Sub-cellular localization | Cytoplasm. Cytoplasm, perinuclear region. Cell projection, growth cone . Note: In neurons, seems to concentrate at axonal growth cone. Perinuclear in neurons (By similarity). |
Sequence Similarities | Belongs to the NDRG family. |
Aliases | Antidepressant related protein ADRG123 antibody|Cytoplasmic protein Ndr1 antibody|DKFZp781G1938 antibody|FLJ25522 antibody|KIAA1248 antibody|N myc downstream regulated gene 2 antibody|N myc downstream regulator 2 antibody|NDR1 related protein NDR2 antibody|NDRG 2 antibody|NDRG family member 2 antibody|NDRG1 related protein antibody|NDRG2 antibody|NDRG2_HUMAN antibody|Protein NDRG2 antibody|Protein Syld709613 antibody|SYLD antibody|Syld709613 protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- NDRG2 antibody, ASA-B1353, Western blotting
All lanes: Anti NDRG2 (ASA-B1353) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: PANC Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
All lanes: Anti NDRG2 (ASA-B1353) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: PANC Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
