More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| nicastrin | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Nicastrin(72-88aa VIHVVEKEEDLQWVLTD), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NCSTN |
Protein | Nicastrin |
Uniprot ID | Q92542 |
Function | Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta- amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex. |
Tissue Specificity | Widely expressed. |
Sub-cellular localization | Membrane ; Single-pass type I membrane protein . Melanosome. |
Sequence Similarities | |
Aliases | Anterior pharynx defective 2 antibody|APH 2 antibody|APH2 antibody|ATAG1874 antibody|KIAA0253 antibody|Ncstn antibody|NCT antibody|NICA_HUMAN antibody|Nicastrin antibody|RP11 517F10.1 antibody|RP11517F101 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NCSTN antibody, ASA-B1351, All Western blotting
All lanes: Anti-NCSTN(ASA-B1351) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Predicted bind size: 78KD
Observed bind size: 78KD
All lanes: Anti-NCSTN(ASA-B1351) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Predicted bind size: 78KD
Observed bind size: 78KD
