More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| mutS homolog 3 | Polyclonal | IgG | Rabbit | Human, Mouse | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human MSH3 recombinant protein (Position: E434-N739). Human MSH3 shares 83% amino acid (aa) sequence identity with mouse MSH3. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MSH3 |
Protein | DNA mismatch repair protein Msh3 |
Uniprot ID | P20585 |
Function | Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion- deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | Belongs to the DNA mismatch repair MutS family. MSH3 subfamily. |
Aliases | Divergent upstream protein antibody|DNA mismatch repair protein antibody|DNA mismatch repair protein Msh 3 antibodyDNA mismatch repair protein Msh3 antibody|DUC 1 antibody|DUC1 antibody|DUG antibody|DUP antibody|hMSH3 antibody|MGC163306 antibody|MGC163308 antibody|Mismatch repair protein 1 antibody|MRP 1 antibody|MRP1 antibody|MSH 3 antibody|MSH3 antibody|MSH3_HUMAN antibody|MutS homolog 3 (E. coli) antibody|MutS homolog 3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- MSH3 antibody, ASA-B1314, Western blotting
All lanes: Anti MSH3 (ASA-B1314) at 0.5ug/ml
Lane 1: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 3: NIH Whole Cell Lysate at 40ug
Lane 4: U20S Whole Cell Lysate at 40ug
Lane 5: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 127KD
Observed bind size: 127KD
All lanes: Anti MSH3 (ASA-B1314) at 0.5ug/ml
Lane 1: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 3: NIH Whole Cell Lysate at 40ug
Lane 4: U20S Whole Cell Lysate at 40ug
Lane 5: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 127KD
Observed bind size: 127KD
