More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| matrix metallopeptidase 9(gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human MMP9(689-705aa NQVDQVGYVTYDILQCP), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MMP9 |
Protein | Matrix metalloproteinase-9(MMP-9) |
Uniprot ID | P14780 |
Function | May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- |
Tissue Specificity | Produced by normal alveolar macrophages and granulocytes. |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix . |
Sequence Similarities | Belongs to the peptidase M10A family. |
Aliases | 82 kDa matrix metalloproteinase-9 antibody|92 kDa gelatinase antibody|92 kDa type IV collagenase antibody|CLG 4B antibody|CLG4B antibody|Collagenase Type 4 beta antibody|Collagenase type IV 92 KD antibody|EC 3.4.24.35 antibody|Gelatinase 92 KD antibody|Gelatinase B antibody|Gelatinase beta antibody|GelatinaseB antibody|GELB antibody|Macrophage gelatinase antibody|MANDP2 antibody|Matrix metallopeptidase 9(gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody|Matrix Metalloproteinase 9 antibody|MMP 9 antibody|MMP-9 antibody|MMP9 antibody|MMP9_HUMAN antibody|Type V collagenase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MMP9 antibody, ASA-B1296, Western blotting
Lane 1: Rat Embryo Tissue Lysate
Lane 2: MM453 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: SMMC Cell Lysate
Lane 5: JURKAT Cell Lysate
Lane 6: HT1080 Cell Lysate
Lane 1: Rat Embryo Tissue Lysate
Lane 2: MM453 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: SMMC Cell Lysate
Lane 5: JURKAT Cell Lysate
Lane 6: HT1080 Cell Lysate
