More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| matrix metallopeptidase 24(membrane-inserted) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human MMP24(208-225aa YHEIKSDRKEADIMIFFA), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MMP24 |
Protein | Matrix metalloproteinase-24 |
Uniprot ID | Q9Y5R2 |
Function | Metalloprotease that mediates cleavage of N-cadherin (CDH2) and acts as a regulator of neuro-immune interactions and neural stem cell quiescence. Involved in cell-cell interactions between nociceptive neurites and mast cells, possibly by mediating cleavage of CDH2, thereby acting as a mediator of peripheral thermal nociception and inflammatory hyperalgesia. Key regulator of neural stem cells quiescence by mediating cleavage of CDH2, affecting CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate their quiescence. May play a role in axonal growth. Able to activate progelatinase A. May also be a proteoglycanase involved in degradation of proteoglycans, such as dermatan sulfate and chondroitin sulfate proteoglycans. Cleaves partially fibronectin, but not collagen type I, nor laminin (By similarity). |
Tissue Specificity | Predominantly expressed in brain, kidney, pancreas and lung. Overexpressed in a series of brain tumors, including astrocytomas and glioblastomas. |
Sub-cellular localization | Matrix metalloproteinase-24: Cell membrane ; Single-pass type I membrane protein . Golgi apparatus, trans-Golgi network membrane ; Single-pass type I membrane protein . Note: Recycled back to the plasma membrane through the trans-Golgi network via interaction with APBA3. |
Sequence Similarities | Belongs to the peptidase M10A family. |
Aliases | Matrix metallopeptidase 24(membrane inserted) antibody|Matrix metallopeptidase 24 antibody|Membrane type 5 matrix metalloproteinase antibody|Membrane type matrix metalloproteinase 5 antibody|Membrane-type matrix metalloproteinase 5 antibody|Membrane-type-5 matrix metalloproteinase antibody|MMP 24 antibody|MMP 25 antibody|MMP-24 antibody|Mmp24 antibody|MMP24_HUMAN antibody|MMP25 antibody|MT MMP5 antibody|MT-MMP 5 antibody|MT5 MMP antibody|MT5-MMP antibody|MT5MMP antibody|MTMMP5 antibody|Processed matrix metalloproteinase-24 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MMP24 antibody, ASA-B1291, Western blotting
Lane 1: PANC Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SMMC Cell Lysate
Lane 4: A549 Cell Lysate
Lane 1: PANC Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SMMC Cell Lysate
Lane 4: A549 Cell Lysate
