More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| nuclear receptor subfamily 3, group C, member 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Mineralocorticoid Receptor(966-984aa DQLPKVESGNAKPLYFHRK), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NR3C2 |
Protein | Mineralocorticoid receptor(MR) |
Uniprot ID | P08235 |
Function | Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels. |
Tissue Specificity | Ubiquitous. Highly expressed in distal tubules, convoluted tubules and cortical collecting duct in kidney, and in sweat glands. Detected at lower levels in cardiomyocytes, in epidermis and in colon enterocytes. |
Sub-cellular localization | Cytoplasm. Nucleus. Endoplasmic reticulum membrane; Peripheral membrane protein. Note: Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane. |
Sequence Similarities | Belongs to the nuclear hormone receptor family. NR3 subfamily. |
Aliases | Aldosterone receptor antibody|MCR antibody|MCR_HUMAN antibody|MGC133092 antibody|Mineralocorticoid receptor antibody|MLR antibody|MR antibody|NR3 C2 antibody|NR3C2 antibody|NR3C2 protein antibody|Nuclear receptor subfamily 3 group C member 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Mineralocorticoid Receptor antibody, ASA-B1278, Western blotting
Lane 1: 293T Cell Lysate
Lane 2: SMMC Cell Lysate
Lane 3: SW620 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 1: 293T Cell Lysate
Lane 2: SMMC Cell Lysate
Lane 3: SW620 Cell Lysate
Lane 4: HELA Cell Lysate
