More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| midkine (neurite growth-promoting factor 2) | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Midkine recombinant protein (Position: V21-D143). Human Midkine shares 87% and 89% amino acid (aa) sequence identity with mouse and rat Midkine, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MDK |
Protein | Midkine |
Uniprot ID | P21741 |
Function | Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development (By similarity). |
Tissue Specificity | Expressed in various tumor cell lines. In insulinoma tissue predominantly expressed in precancerous lesions. |
Sub-cellular localization | Secreted. |
Sequence Similarities | |
Aliases | Amphiregulin associated protein antibody|Amphiregulin-associated protein antibody|ARAP antibody|FLJ27379 antibody|MDK antibody|Midgestation and kidney protein antibody|Midkine antibody|MK 1 antibody|MK antibody|MK_HUMAN antibody|MK1 antibody|NEGF 2 antibody|NEGF2 antibody|neurite growth promoting factor 2 antibody|Neurite outgrowth promoting protein antibody|Neurite outgrowth-promoting factor 2 antibody|Neurite outgrowth-promoting protein antibody|Retinoic acid inducible factor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Midkine antibody, ASA-B1274, Western blotting
All lanes: Anti Midkine (ASA-B1274) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 16KD
Observed bind size: 38KD
All lanes: Anti Midkine (ASA-B1274) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 16KD
Observed bind size: 38KD
