More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| multiple endocrine neoplasia I | Polyclonal | IgG | Rabbit | Human, Mouse | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Menin recombinant protein (Position: P301-L615). Human Menin shares 93% and 94% amino acid (aa) sequence identity with mouse and rat Menin, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MEN1 |
Protein | Menin |
Uniprot ID | O00255 |
Function | Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression (By similarity). May be involved in DNA repair. |
Tissue Specificity | Ubiquitous. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | |
Aliases | MEA 1 antibody|MEA1 antibody|MEN 1 antibody|MEN1 antibody|MEN1_HUMAN antibody|Menin antibody|Multiple Endocrine Adenomatosis 1 antibody|Multiple Endocrine Neoplasia 1 antibody|SCG 2 antibody|SCG2 antibody|Suppressor Candidate Gene 2 antibody|Wermer syndrome antibody|ZES antibody|Zollinger Ellison Syndrome antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Menin antibody, ASA-B1263, Western blotting
All lanes: Anti Menin (ASA-B1263) at 0.5ug/ml
WB: Recombinant Human Menin Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti Menin (ASA-B1263) at 0.5ug/ml
WB: Recombinant Human Menin Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti- Menin antibody, ASA-B1263, Western blotting
All lanes: Anti Menin (ASA-B1263) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: 293T Whole Cell Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Lane 4: HEPA Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 68KD
Observed bind size: 68KD
All lanes: Anti Menin (ASA-B1263) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: 293T Whole Cell Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Lane 4: HEPA Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 68KD
Observed bind size: 68KD
