More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| mitogen-activated protein kinase kinase kinase 3 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human MEKK3(11-25aa MNDLVALQMNRRHRM). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MAP3K3 |
Protein | Mitogen-activated protein kinase kinase kinase 3 |
Uniprot ID | Q99759 |
Function | Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators. |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. |
Aliases | M3K3_HUMAN antibody|MAP/ERK kinase kinase 3 antibody|MAP3K3 antibody|MAPK/ERK kinase kinase 3 antibody|MAPKKK3 antibody|MEK kinase 3 antibody|MEKK 3 antibody|MEKK3 antibody|mitogen activated protein kinase kinase kinase 3 antibody|Mitogen-activated protein kinase kinase kinase 3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MEKK3 antibody, ASA-B1261, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: A431 Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: A431 Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: JURKAT Cell Lysate
