More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| mitogen-activated protein kinase kinase 6 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human MEK6(7-23aa KKRNPGLKIPKEAFEQP), identical to the related mouse sequence and different from the related rat sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MAP2K6 |
Protein | Dual specificity mitogen-activated protein kinase kinase 6 |
Uniprot ID | P52564 |
Function | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG- dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases. |
Tissue Specificity | Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. |
Aliases | Dual specificity mitogen activated protein kinase kinase 6 antibody|Dual specificity mitogen-activated protein kinase kinase 6 antibody|EC 2.7.12.2 antibody|MAP kinase kinase 6 antibody|MAP2K6 antibody|MAPK/ERK kinase 6 antibody|MAPKK 6 antibody|MAPKK6 antibody|MEK 6 antibody|MEK6 antibody|Mitogen Activated Protein Kinase Kinase 6 antibody|MKK 6 antibody|MKK6 antibody|MP2K6_HUMAN antibody|PRKMK6 antibody|Protein kinase mitogen activated kinase 6 antibody|protein kinase, mitogen-activated, kinase 6(MAP kinase kinase 6) antibody|SAPK kinase 3 antibody|SAPKK-3 antibody|SAPKK3 antibody|SKK3 antibody|Stress-activated protein kinase kinase 3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MEK6 antibody, ASA-B1257, Western blotting
Lane 1: Rat Ovary Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Lane 4: Rat Heart Tissue Lysate
Lane 5: NIH3I3 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: JRUKAT Cell Lysate
Lane 8: MCF-7 Cell Lysate
Lane 1: Rat Ovary Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Lane 4: Rat Heart Tissue Lysate
Lane 5: NIH3I3 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: JRUKAT Cell Lysate
Lane 8: MCF-7 Cell Lysate
