More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| mitogen-activated protein kinase kinase 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human MEK4(360-378aa ELLKHPFILMYEERAVEVA), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MAP2K4 |
Protein | Dual specificity mitogen-activated protein kinase kinase 4 |
Uniprot ID | P45985 |
Function | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14. |
Tissue Specificity | Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. |
Sub-cellular localization | Cytoplasm . Nucleus . |
Sequence Similarities | Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. |
Aliases | c Jun N terminal kinase kinase 1 antibody|C-JUN N-terminal kinase kinase 1 antibody|Dual specificity mitogen activated protein kinase kinase 4 antibody|Dual specificity mitogen-activated protein kinase kinase 4 antibody|JNK Activated Kinase 1 antibody|JNK activating kinase 1 antibody|JNK-activating kinase 1 antibody|JNKK antibody|JNKK1 antibody|MAP kinase kinase 4 antibody|Map2k4 antibody|MAPK ERK kinase 4 antibody|MAPK/ERK kinase 4 antibody|MAPKK 4 antibody|MAPKK4 antibody|MEK 4 antibody|MEK4 antibody|Mitogen activated protein kinase kinase 4 antibody|MKK 4 antibody|MKK4 antibody|PRKMK4 antibody|SAPK ERK kinase 1 antibody|SAPK/ERK kinase 1 antibody|SEK1 antibody|SERK1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MEK4 antibody, ASA-B1256, All Western blotting
All lanes: Anti-MAP2K4(ASA-B1256) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: CEM Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 44KD
All lanes: Anti-MAP2K4(ASA-B1256) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: CEM Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 44KD
