More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| mitogen-activated protein kinase kinase 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human MEK2(2-20aa LARRKPVLPALTINPTIAE), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MAP2K2 |
Protein | Dual specificity mitogen-activated protein kinase kinase 2 |
Uniprot ID | P36507 |
Function | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity). |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. |
Aliases | Cardiofaciocutaneous syndrome antibody|CFC syndrome antibody|CFC4 antibody|Dual specificity mitogen activated protein kinase kinase 2 antibody|Dual specificity mitogen-activated protein kinase kinase 2 antibody|ERK activator kinase 2 antibody|FLJ26075 antibody|MAP kinase kinase 2 antibody|MAP2K 2 antibody|map2k2 antibody|MAPK / ERK kinase 2 antibody|MAPK/ERK kinase 2 antibody|MAPKK 2 antibody|MAPKK2 antibody|MEK 2 antibody|MEK2 antibody|Microtubule Associated Protein Kinase Kinase 2 antibody|Mitogen activated protein kinase kinase 2 antibody|Mitogen activated protein kinase kinase 2 p45 antibody|MKK 2 antibody|MKK2 antibody|OTTHUMP00000165826 antibody|OTTHUMP00000165827 antibody|PRKMK 2 antibody|PRKMK2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MEK2 antibody, ASA-B1253, All Western blotting
All lanes: MAP2K2(ASA-B1253) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug
Lane 2: Rat Kidney Tissue Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: PC12 Whole Cell Lysate at 40ug
Lane 6: JURKAT Whole Cell Lysate at 40ug
Lane 7: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 50KD
Observed bind size: 50KD
All lanes: MAP2K2(ASA-B1253) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug
Lane 2: Rat Kidney Tissue Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: PC12 Whole Cell Lysate at 40ug
Lane 6: JURKAT Whole Cell Lysate at 40ug
Lane 7: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 50KD
Observed bind size: 50KD
